Publications from Spiker Lab

63. Halweg, C., Thompson, W. F., and Spiker, S. (2005) The Rb7 matrix attachment region increases the
          likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric study. Plant  Cell 17,
          418-429 (pdf)

62. Francis, K.E. and Spiker, S. (2005) Identification of Arabidopsis transformants without selection  reveals a high
          occurrence of silenced T-DNA integrations. The Plant Journal 41, 464-477 (pdf)

61. Allen, G. C., Spiker, S., and Thompson, W. F. (2005) Transgene integration: use of matrix attachment
          regions. Methods Mol Biol 286, 313-326

60. Busov, V.B., Johannes, E., Whetten, R.W., Sederoff, R.R., Spiker, S., Lanz-Garcia, C. and Goldfarb, B.
          (2004) An auxin-inducible gene from loblolly pine ( Pinus taeda L.) is differentially expressed in mature and
          juvenile phase shoots and encodes a putative transmembrane protein. Planta 218,  916-927

59. Mankin, S.L., Allen, G.C., Phelan, T., Spiker, S. and Thompson, W.F. (2003) Elevation of transgene
          expression level by flanking matrix attachment regions (MAR) is promoter dependent: A study of the
          interactions of six promoters with the RB7 3' MAR. Transgenic Research 12, 3-12 (pdf)

58. Ülker, B., Weissinger, A.K. and Spiker, S. (2002) E. coli chromosomal DNA in a transgene locus created by
          microprojectile bombardment. Transgenic Research 11, 311-313 (pdf)

57. Ascenzi, R., Ingram, J.L., Massel, M., Thompson, W.F., Spiker, S. and Weissinger, A.K. (2001) The role of
          cell differentiation state and HMGI/Y in the expression of transgenes flanked by matrix attachment regions.
          Transgenic Research 10, 465-470 (pdf)

56. Mendu, N., Massel, M. and Spiker, S. (2001) Increasing loop domain size does not diminish effects of matrix
          attachment regions on transgene expression in tobacco cells in culture. FEBS Lett. 496, 66-67 (pdf)

55. Allen, G.C., Spiker, S. and Thompson, W.F. (2000) Use of matrix attachment regions (MARs) to minimize
          transgene silencing. Plant Mol. Biol. 43, 361-376 (pdf)

54. Michalowski, S.M., Allen, G.C., Hall, G.E., Jr., Thompson, W.F. and Spiker, S. (1999) Characterization of
          randomly-obtained matrix attachment regions (MARs) from higher plants. Biochemistry 38, 12795-12804
          (pdf)

53. Ülker, B., Allen, G.C., Thompson, W.F., Spiker, S. and Weissinger, A.K. (1999) A tobacco matrix attachment
          region reduces the loss of transgene expression in the progeny of transgenic tobacco plants.  The Plant
          Journal 18, 253-264 (pdf)

52. Meier, I., Groning, B., Michalowski, S. and Spiker, S. (1997) The tomato RBCS3A promoter requires
          integration into the chromatin for correct organ-specific regulation. FEBS Lett. 415, 91-95

51. Iglesias, V.A., Moscone, E.A., Papp, I., Michalowski, S., Phelan, T., Spiker, S., Neuhuber, F., Matzke, M.
          and Matzke, A.J.M. (1997) Molecular and cytogenetic analysis of stably and unstably expressed transgene
          loci in tobacco. Plant Cell 9, 1251-1264

50. Meier, I., Phelan, T., Gruissem, W., Spiker, S. and Schneider, D. (1996) MFP1, a novel plant filament-like
          protein with affinity for matrix attachment region DNA. Plant Cell 8, 2105-2115

49. Schultz, T.F., Spiker, S. and Quatrano, R.S. (1996) Histone H1 enhances the DNA binding activity of the
          transcription factor EmBP-1. J. Biol. Chem. 271, 25742-25745

48. Prymakowska-Bosak, M., Przewloka, M., Iwkiewicz, J., Egierszdorff, S., Kuras, M., Chaubet, N., Gigot, C.,
          Spiker, S. and Jerzmanowski, A. (1996) Histone H1 overexpressed to high level in tobacco affects certain
          developmental programs but has limited effect on basal cellular functions. Proc. Natl. Acad. Sci. USA 93,
          10250-10255

47. Papp, I., Iglesias, V.A., Moscone, E.A., Michalowski, S., Spiker, S., Park, Y.-D., Matzke, M.A. and Matzke,
          A.J.M. (1996) Structural instability of a transgene locus in tobacco is associated with aneuploidy. The Plant
          Journal 10, 469-478 

46. Allen, G.C., Hall, G., Jr., Michalowski, S., Newman, W., Weissinger, A.K., Spiker, S. and Thompson, W.F.
          (1996) High level transgene expression in plant cells: Effects of a strong SAR from tobacco. Plant Cell 8,
          899-913 (pdf)

45. Thompson, W.F., Allen, G.C., Hall, G., Jr. and Spiker, S. (1996) Matrix attachment regions and transgene
          expression. In: Genomes of Plants and Animals (Proceedings of the 21st Stadler Genetics Symposium,
          Gustafson, P. and Flavell, R.B. eds.) pp 243-270 Plenum Publishing Company, New York.

44. Spiker, S. and Thompson, W.F. (1996) Nuclear Matrix Attachment Regions and transgene expression in plants.
          Plant Physiol. 110, 15-21 (pdf)

43. Thompson, W.F., Allen, G.C., Hall, G., Michalowski, S., Newman, W., Spiker, S. and Weissinger, A. (1995)
          A nuclear scaffold attachment region from tobacco greatly increases transgene expression in plant cells. In:
          Modification of Gene Expression and Non-Mendelian Inheritance, Oono, K. and Takaiwa, F. eds.
          pp 281-294

42. van der Geest, A.H.M., Hall, G.E., Jr., Spiker, S. and Hall, T.C. (1994) The ß-phaseolin gene is flanked by
          matrix attachment regions. The Plant Journal 6, 413-423

41. Gigot, C. and Spiker, S. (1994) Nomenclature of genes encoding histones. Plant Molecular Biology Reporter
          12, S39-40

40. Hall, G.E., Jr. and Spiker, S. (1994) Isolation and characterization of nuclear scaffolds. Plant Molecular Biology
          Manual (eds. S. Gelvin, R. Schilperoort and D.P. Verma) pp. D2:1-12 Kluwer Academic Publishers,
          Dordrecht, The Netherlands

39. Allen, G.C., Hall, G.E., Jr., Childs, L.C., Weissinger, A.K., Spiker, S. and Thompson, W.F. (1993) Scaffold
          attachment regions (SARs) increase reporter gene expression in stably transformed plant cells. Plant Cell 5,
          603-613 (pdf)

38. Moehs, C.P., Baxevanis, A.D., Moudrianakis, E.N. and Spiker, S. (1992) Enhanced stability of histone
          octamers from plant nucleosomes: Role of H2A and H2B histones. Biochemistry 31, 10844-10851 (pdf)

37. Loer, D.S. and Spiker, S. (1992) Chromatin structure of heat shock-inducible and abscisic acid-inducible genes
          in wheat. Physiologia Plantarum 86, 77-84

36. McElwain, E.F. and Spiker, S. (1992) Molecular and physiological analysis of a heat-shock response in wheat.
          Plant Physiology 99, 1455-1460

35. Hall, G., Jr., Allen, G.C., Loer, D.S., Thompson, W.F. and Spiker, S. (1991) Nuclear scaffolds and scaffold
          attachment regions in higher plants. Proc. Natl. Acad. Sci. 88, 9320-9324 (pdf)

34. Arwood, L.A., Hill, E. and Spiker, S. (1991) Ethidium bromide preferentially releases high mobility group
          chromosomal proteins HMGc and HMGd from wheat embryo nuclei. Physiol. Plant. 82, 419-422

33. Pedersen, T.J., Arwood, L.J., Spiker, S., Guiltinan, M.J. and Thompson, W.F. (1991) High mobility group
          chromosomal proteins bind to AT-rich tracts flanking plant genes. Plant Molec. Biol. 16, 95-104

32. Spiker, S., Weisshaar, B., de Costa e Silva, O. and Hahlbrock, K.(1990) Sequence of a histone H2A cDNA
          from Parsley. Nucleic Acids Res. 18, 5897

31. Arwood, L.A. and Spiker, S. (1990) Binding of wheat and chicken High Mobility Group chromosomal proteins
          to DNA and to wheat and chicken mononucleosomes. J. Biol. Chem. 265, 9771-9777 (pdf)

30. Spiker, S. (1990) A vertical submarine electrophoresis apparatus for polyacrylamide minigels. Anal. Biochem.
          185, 270-273

29. McElwain, E.F. and Spiker, S. (1989) A wheat cDNA clone which is homologous to the 17 kd heat-shock
          protein gene family of soybean. Nuc. Acids Res. 17, 1764

28. Moehs, C.P., McElwain, E.F. and Spiker, S. (1988) Chromosomal proteins of Arabidopsis thaliana. Plant
          Molec. Biology 11, 507-515

27. Spiker, S. (1988) Histone variants and high mobility group non-histone chromosomal proteins of higher plants:
          Their potential for forming a chromatin structure that is either poised for transcription or transcriptionally inert.
          Physiologia Plantarum 74, 200-213

26. Spiker, S. (1988) Histones and HMG proteins of higher plants. In: Architecture of Eukaryotic Genes: Kaul, G.
          (ed.) VCH Verlagsgesellschaft, Weinheim, Federal Republic of Germany, pp. 143-162

25. Spiker, S., Hopkins, R., Fischer, R. and Quatrano, R.S. (1987) Synthesis of nucleosomal histone variants
          during wheat grain development. Biochim. Biophys. Acta 910, 157-162

24. Spiker, S. and Everett, K.M. (1987) Blotting Index of Dissimilarity: Use to study immunological relatedness of
          plant and animal high mobility group (HMG) chromosomal proteins. Plant Molec. Biol. 9, 431-442

23. Spiker, S., Bates, M. and Arwood, L.J. (1987) Fractionation of plant and animal high mobility group
          chromosomal proteins by ion-exchange and reversed-phase high-performance liquid chromatography. J.
          Chromatog. 405, 327-336

22. Spiker, S. (1985) Plant chromatin structure. Annu. Rev. Plant Physiol. 36, 235-253 (pdf)

21. Spiker, S. (1984) High mobility group proteins of wheat. J. Biol. Chem. 259, 12007-12013 (pdf)

20. Spiker, S. (1984) Chromatin structure and gene regulation in higher plants. Advances in Genetics 22, 145-208

19. Spiker, S. and Isenberg, I. (1983) Preparative polyacrylamide gel electrophoresis. Methods in Enzymology 91,
          214-226

18. Spiker, S., Murray, M. G. and Thompson, W. F. (1983) DNase I sensitivity of transcriptionally active genes in
          intact nuclei and isolated chromatin of plants. Proc. Natl. Acad. Sci. USA 80, 815-819 (pdf)

17. Spiker, S. (1982) Histone variants in plants: evidence for primary structure variants differing in molecular weight.
          J. Biol. Chem. 257, 14250-14255 (pdf)

16. Spiker, S. (1980) A slab gel designed for enzymatic digestion of proteins in polyacrylamide gel slices and direct
          resolution of peptides. J. Chromatog. 198, 169-171

15. Spiker, S. (1980) A modification of the acetic acid-urea system for use in microslab polyacrylamide gel
          electrophoresis. Anal. Biochem. 108, 263-265

14. Spiker, S. and Isenberg, I. (1978) Evolutionary conservation of histone-histone binding sites: Evidence from
          interkingdom complex formation. Cold Spring Harbor Symp. Quant. Biol. 42, 157-163

13. Spiker, S., Mardian, J.K.W. and Isenberg, I. (1978) Chromosomal HMG proteins occur in three eukaryotic
          kingdoms. Biochem. Biophys. Res. Commun. 82, 129-135

12. Isenberg, I. and Spiker, S. (1977) The approach to a functional definition of histones: A current stock taking.
          Human Cytogenetics: ICN UCLA Symposia on Molecular and Cellular Biology 7, 17-23. Academic Press,
          New York, NY

11. Spiker, S. and Isenberg, I. (1977) The cross-complexing pattern of plant histones.
          Biochemistry 16, 1819-1826 (pdf)

10. Spiker, S., Key, J. L. and Wakim, B. (1976) Identification and fractionation of plant histones. Arch. Biochem.
          Biophys. 176, 510-518

9. Spiker, S. (1976) Identification of histones F2b and F1 in stained gels. J. Chromatog. 128, 244-248

8. Spiker, S., Mashkas, A. and Muazzaz, Y. (1976) The effect of single and repeated gibberellic acid treatment on
          internode number and length in dwarf peas. Physiologia Plantarum 36, 1-3

7. Spiker, S. (1976) Expression of parental histone genes in the intergeneric hybrid Triticale hexaploide. Nature
           259, 418-420  (pdf)

6. Spiker, S. (1975) An evolutionary comparison of plant histones. Biochimica et Biophysica Acta 400, 461-467

5. Spiker, S. and Krishnaswamy, L. (1973) Constancy of wheat histones during development. Planta 110, 71-76

4. Spiker, S. and Chalkley, R. (1972) Evidence against an effect of plant hormones on thermal denaturation of pea           nucleoprotein. Planta 102, 362-367

3. Oliver, D., Sommer, K., Panyim, S., Spiker, S. and Chalkley, R. (1972) A modified procedure for fractionating
          histones. Biochemical Journal 129, 349-353

2. Spiker, S. and Chalkley, R. (1971) Electrophoretic analysis of histones from gibberellic acid-treated dwarf peas.
          Plant Physiology 47, 342-345

1. Panyim, S., Chalkley, R., Spiker, S. and Oliver, D. (1971) Constant electrophoretic mobility of the cysteine-
          containing histone in and animals. Biochimica et Biophysica Acta 214, 216-221