Publications from Spiker Lab |
63. Halweg, C., Thompson, W. F., and Spiker, S. (2005) The Rb7 matrix attachment region increases the
likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric study. Plant Cell 17,
418-429 (pdf)
62. Francis, K.E. and Spiker, S. (2005) Identification of Arabidopsis transformants without selection reveals a high
occurrence of silenced T-DNA integrations. The Plant Journal 41, 464-477 (pdf)
61. Allen, G. C., Spiker, S., and Thompson, W. F. (2005) Transgene integration: use of matrix attachment
regions. Methods Mol Biol 286, 313-326
60. Busov, V.B., Johannes, E., Whetten, R.W., Sederoff, R.R., Spiker, S., Lanz-Garcia, C. and Goldfarb, B.
(2004) An auxin-inducible gene from loblolly pine ( Pinus taeda L.) is differentially expressed in mature and
juvenile phase shoots and encodes a putative transmembrane protein. Planta 218, 916-927
59. Mankin, S.L., Allen, G.C., Phelan, T., Spiker, S. and Thompson, W.F. (2003) Elevation of transgene
expression level by flanking matrix attachment regions (MAR) is promoter dependent: A study of the
interactions of six promoters with the RB7 3' MAR. Transgenic Research 12, 3-12 (pdf)
58. Ülker, B., Weissinger, A.K. and Spiker, S. (2002) E. coli chromosomal DNA in a transgene locus created by
microprojectile bombardment. Transgenic Research 11, 311-313 (pdf)
57. Ascenzi, R., Ingram, J.L., Massel, M., Thompson, W.F., Spiker, S. and Weissinger, A.K. (2001) The role of
cell differentiation state and HMGI/Y in the expression of transgenes flanked by matrix attachment regions.
Transgenic Research 10, 465-470 (pdf)
56. Mendu, N., Massel, M. and Spiker, S. (2001) Increasing loop domain size does not diminish effects of matrix
attachment regions on transgene expression in tobacco cells in culture. FEBS Lett. 496, 66-67 (pdf)
55. Allen, G.C., Spiker, S. and Thompson, W.F. (2000) Use of matrix attachment regions (MARs) to minimize
transgene silencing. Plant Mol. Biol. 43, 361-376 (pdf)
54. Michalowski, S.M., Allen, G.C., Hall, G.E., Jr., Thompson, W.F. and Spiker, S. (1999) Characterization of
randomly-obtained matrix attachment regions (MARs) from higher plants. Biochemistry 38, 12795-12804
(pdf)
53. Ülker, B., Allen, G.C., Thompson, W.F., Spiker, S. and Weissinger, A.K. (1999) A tobacco matrix attachment
region reduces the loss of transgene expression in the progeny of transgenic tobacco plants. The Plant
Journal 18, 253-264 (pdf)
52. Meier, I., Groning, B., Michalowski, S. and Spiker, S. (1997) The tomato RBCS3A promoter requires
integration into the chromatin for correct organ-specific regulation. FEBS Lett. 415, 91-95
51. Iglesias, V.A., Moscone, E.A., Papp, I., Michalowski, S., Phelan, T., Spiker, S., Neuhuber, F., Matzke, M.
and Matzke, A.J.M. (1997) Molecular and cytogenetic analysis of stably and unstably expressed transgene
loci in tobacco. Plant Cell 9, 1251-1264
50. Meier, I., Phelan, T., Gruissem, W., Spiker, S. and Schneider, D. (1996) MFP1, a novel plant filament-like
protein with affinity for matrix attachment region DNA. Plant Cell 8, 2105-2115
49. Schultz, T.F., Spiker, S. and Quatrano, R.S. (1996) Histone H1 enhances the DNA binding activity of the
transcription factor EmBP-1. J. Biol. Chem. 271, 25742-25745
48. Prymakowska-Bosak, M., Przewloka, M., Iwkiewicz, J., Egierszdorff, S., Kuras, M., Chaubet, N., Gigot, C.,
Spiker, S. and Jerzmanowski, A. (1996) Histone H1 overexpressed to high level in tobacco affects certain
developmental programs but has limited effect on basal cellular functions. Proc. Natl. Acad. Sci. USA 93,
10250-10255
47. Papp, I., Iglesias, V.A., Moscone, E.A., Michalowski, S., Spiker, S., Park, Y.-D., Matzke, M.A. and Matzke,
A.J.M. (1996) Structural instability of a transgene locus in tobacco is associated with aneuploidy. The Plant
Journal 10, 469-478
46. Allen, G.C., Hall, G., Jr., Michalowski, S., Newman, W., Weissinger, A.K., Spiker, S. and Thompson, W.F.
(1996) High level transgene expression in plant cells: Effects of a strong SAR from tobacco. Plant Cell 8,
899-913 (pdf)
45. Thompson, W.F., Allen, G.C., Hall, G., Jr. and Spiker, S. (1996) Matrix attachment regions and transgene
expression. In: Genomes of Plants and Animals (Proceedings of the 21st Stadler Genetics Symposium,
Gustafson, P. and Flavell, R.B. eds.) pp 243-270 Plenum Publishing Company, New York.
44. Spiker, S. and Thompson, W.F. (1996) Nuclear Matrix Attachment Regions and transgene expression in plants.
Plant Physiol. 110, 15-21 (pdf)
43. Thompson, W.F., Allen, G.C., Hall, G., Michalowski, S., Newman, W., Spiker, S. and Weissinger, A. (1995)
A nuclear scaffold attachment region from tobacco greatly increases transgene expression in plant cells. In:
Modification of Gene Expression and Non-Mendelian Inheritance, Oono, K. and Takaiwa, F. eds.
pp 281-294
42. van der Geest, A.H.M., Hall, G.E., Jr., Spiker, S. and Hall, T.C. (1994) The ß-phaseolin gene is flanked by
matrix attachment regions. The Plant Journal 6, 413-423
41. Gigot, C. and Spiker, S. (1994) Nomenclature of genes encoding histones. Plant Molecular Biology Reporter
12, S39-40
40. Hall, G.E., Jr. and Spiker, S. (1994) Isolation and characterization of nuclear scaffolds. Plant Molecular Biology
Manual (eds. S. Gelvin, R. Schilperoort and D.P. Verma) pp. D2:1-12 Kluwer Academic Publishers,
Dordrecht, The Netherlands
39. Allen, G.C., Hall, G.E., Jr., Childs, L.C., Weissinger, A.K., Spiker, S. and Thompson, W.F. (1993) Scaffold
attachment regions (SARs) increase reporter gene expression in stably transformed plant cells. Plant Cell 5,
603-613 (pdf)
38. Moehs, C.P., Baxevanis, A.D., Moudrianakis, E.N. and Spiker, S. (1992) Enhanced stability of histone
octamers from plant nucleosomes: Role of H2A and H2B histones. Biochemistry 31, 10844-10851 (pdf)
37. Loer, D.S. and Spiker, S. (1992) Chromatin structure of heat shock-inducible and abscisic acid-inducible genes
in wheat. Physiologia Plantarum 86, 77-84
36. McElwain, E.F. and Spiker, S. (1992) Molecular and physiological analysis of a heat-shock response in wheat.
Plant Physiology 99, 1455-1460
35. Hall, G., Jr., Allen, G.C., Loer, D.S., Thompson, W.F. and Spiker, S. (1991) Nuclear scaffolds and scaffold
attachment regions in higher plants. Proc. Natl. Acad. Sci. 88, 9320-9324 (pdf)
34. Arwood, L.A., Hill, E. and Spiker, S. (1991) Ethidium bromide preferentially releases high mobility group
chromosomal proteins HMGc and HMGd from wheat embryo nuclei. Physiol. Plant. 82, 419-422
33. Pedersen, T.J., Arwood, L.J., Spiker, S., Guiltinan, M.J. and Thompson, W.F. (1991) High mobility group
chromosomal proteins bind to AT-rich tracts flanking plant genes. Plant Molec. Biol. 16, 95-104
32. Spiker, S., Weisshaar, B., de Costa e Silva, O. and Hahlbrock, K.(1990) Sequence of a histone H2A cDNA
from Parsley. Nucleic Acids Res. 18, 5897
31. Arwood, L.A. and Spiker, S. (1990) Binding of wheat and chicken High Mobility Group chromosomal proteins
to DNA and to wheat and chicken mononucleosomes. J. Biol. Chem. 265, 9771-9777 (pdf)
30. Spiker, S. (1990) A vertical submarine electrophoresis apparatus for polyacrylamide minigels. Anal. Biochem.
185, 270-273
29. McElwain, E.F. and Spiker, S. (1989) A wheat cDNA clone which is homologous to the 17 kd heat-shock
protein gene family of soybean. Nuc. Acids Res. 17, 1764
28. Moehs, C.P., McElwain, E.F. and Spiker, S. (1988) Chromosomal proteins of Arabidopsis thaliana. Plant
Molec. Biology 11, 507-515
27. Spiker, S. (1988) Histone variants and high mobility group non-histone chromosomal proteins of higher plants:
Their potential for forming a chromatin structure that is either poised for transcription or transcriptionally inert.
Physiologia Plantarum 74, 200-213
26. Spiker, S. (1988) Histones and HMG proteins of higher plants. In: Architecture of Eukaryotic Genes: Kaul, G.
(ed.) VCH Verlagsgesellschaft, Weinheim, Federal Republic of Germany, pp. 143-162
25. Spiker, S., Hopkins, R., Fischer, R. and Quatrano, R.S. (1987) Synthesis of nucleosomal histone variants
during wheat grain development. Biochim. Biophys. Acta 910, 157-162
24. Spiker, S. and Everett, K.M. (1987) Blotting Index of Dissimilarity: Use to study immunological relatedness of
plant and animal high mobility group (HMG) chromosomal proteins. Plant Molec. Biol. 9, 431-442
23. Spiker, S., Bates, M. and Arwood, L.J. (1987) Fractionation of plant and animal high mobility group
chromosomal proteins by ion-exchange and reversed-phase high-performance liquid chromatography. J.
Chromatog. 405, 327-336
22. Spiker, S. (1985) Plant chromatin structure. Annu. Rev. Plant Physiol. 36, 235-253 (pdf)
21. Spiker, S. (1984) High mobility group proteins of wheat. J. Biol. Chem. 259, 12007-12013 (pdf)
20. Spiker, S. (1984) Chromatin structure and gene regulation in higher plants. Advances in Genetics 22, 145-208
19. Spiker, S. and Isenberg, I. (1983) Preparative polyacrylamide gel electrophoresis. Methods in Enzymology 91,
214-226
18. Spiker, S., Murray, M. G. and Thompson, W. F. (1983) DNase I sensitivity of transcriptionally active genes in
intact nuclei and isolated chromatin of plants. Proc. Natl. Acad. Sci. USA 80, 815-819 (pdf)
17. Spiker, S. (1982) Histone variants in plants: evidence for primary structure variants differing in molecular weight.
J. Biol. Chem. 257, 14250-14255 (pdf)
16. Spiker, S. (1980) A slab gel designed for enzymatic digestion of proteins in polyacrylamide gel slices and direct
resolution of peptides. J. Chromatog. 198, 169-171
15. Spiker, S. (1980) A modification of the acetic acid-urea system for use in microslab polyacrylamide gel
electrophoresis. Anal. Biochem. 108, 263-265
14. Spiker, S. and Isenberg, I. (1978) Evolutionary conservation of histone-histone binding sites: Evidence from
interkingdom complex formation. Cold Spring Harbor Symp. Quant. Biol. 42, 157-163
13. Spiker, S., Mardian, J.K.W. and Isenberg, I. (1978) Chromosomal HMG proteins occur in three eukaryotic
kingdoms. Biochem. Biophys. Res. Commun. 82, 129-135
12. Isenberg, I. and Spiker, S. (1977) The approach to a functional definition of histones: A current stock taking.
Human Cytogenetics: ICN UCLA Symposia on Molecular and Cellular Biology 7, 17-23. Academic Press,
New York, NY
11. Spiker, S. and Isenberg, I. (1977) The cross-complexing pattern of plant histones.
Biochemistry 16, 1819-1826 (pdf)
10. Spiker, S., Key, J. L. and Wakim, B. (1976) Identification and fractionation of plant histones. Arch. Biochem.
Biophys. 176, 510-518
9. Spiker, S. (1976) Identification of histones F2b and F1 in stained gels. J. Chromatog. 128, 244-248
8. Spiker, S., Mashkas, A. and Muazzaz, Y. (1976) The effect of single and repeated gibberellic acid treatment on
internode number and length in dwarf peas. Physiologia Plantarum 36, 1-3
7. Spiker, S. (1976) Expression of parental histone genes in the intergeneric hybrid Triticale hexaploide. Nature
259, 418-420 (pdf)
6. Spiker, S. (1975) An evolutionary comparison of plant histones. Biochimica et Biophysica Acta 400, 461-467
5. Spiker, S. and Krishnaswamy, L. (1973) Constancy of wheat histones during development. Planta 110, 71-76
4. Spiker, S. and Chalkley, R. (1972) Evidence against an effect of plant hormones on thermal denaturation of pea nucleoprotein. Planta 102, 362-367
3. Oliver, D., Sommer, K., Panyim, S., Spiker, S. and Chalkley, R. (1972) A modified procedure for fractionating
histones. Biochemical Journal 129, 349-353
2. Spiker, S. and Chalkley, R. (1971) Electrophoretic analysis of histones from gibberellic acid-treated dwarf peas.
Plant Physiology 47, 342-345
1. Panyim, S., Chalkley, R., Spiker, S. and Oliver, D. (1971) Constant electrophoretic mobility of the cysteine-
containing histone in and animals. Biochimica et Biophysica Acta 214, 216-221