Arabidopsis 2010: Functional Analysis and Phosphorylation Site Mapping of Leucine-Rich Repeat Receptor-Like Kinases in Arabidopsis

National Science Foundation (MCB)0419819

Project Publications

The following is a list of publications acknowledging the NSF 2010 award. Click on the title for a link to the PDF file where available.

Gou, X, He, K, Yang, H, T., Y, Lin, H, Clouse, SD, and Li, J. Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana. BMC Genomics, 2010. In press.

Oh, MH, Wang, X, Kota, U, Goshe, MB, Clouse, SD, and Huber, SC. Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A, 2009. 106:658-663.

Mitra, SK, Walters, BT, Clouse, SD, and Goshe, MB. An efficient organic solvent based extraction method for the proteomic analysis of Arabidopsis plasma membranes. J Proteome Res, 2009. 8:2752-2767.

Wang, X, Kota, U, He, K, Blackburn, K, Li, J, Goshe, MB, Huber, SC, and Clouse, SD. Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling. Dev Cell, 2008. 15:220-235.

He, K, Gou, X, Yuan, T, Lin, H, Asami, T, Yoshida, S, Russell, SD, and Li, J. BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr Biol, 2007. 17:1109-1115.

Mitra, SK, Gantt, JA, Ruby, JF, Clouse, SD, and Goshe, MB. Membrane proteomic analysis of Arabidopsis thaliana using alternative solubilization techniques. J Proteome Res, 2007. 6:1933-1950.

Yuan, T, Fujioka, S, Takatsuto, S, Matsumoto, S, Gou, X, He, K, Russell, SD, and Li, J. BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana. Plant J, 2007. 51:220-233.

Wang, X, Goshe, MB, Soderblom, EJ, Phinney, BS, Kuchar, JA, Li, J, Asami, T, Yoshida, S, Huber, SC, and Clouse, SD. Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase. Plant Cell, 2005. 17:1685-1703.

Ehsan, H, Ray, WK, Phinney, B, Wang, X, Huber, SC, and Clouse, SD. Interaction of Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase with a homolog of mammalian TGF-beta receptor interacting protein. Plant J, 2005. 43:251-261.

Kota, U, Chien, KY, and Goshe, MB. Isotope-labeling and affinity enrichment of phosphopeptides for proteomic analysis using liquid chromatography-tandem mass spectrometry. Methods Mol Biol, 2009. 564:303-321.

Mitra, SK and Goshe, MB. Cysteinyl-tagging of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry. Methods Mol Biol, 2009. 528:311-326.

Oh, MH, Clouse, SD, and Huber, SC. Tyrosine phosphorylation in brassinosteroid signaling. Plant Signal Behav, 2009. 4.

Blackburn, K and Goshe, MB. Challenges and strategies for targeted phosphorylation site identification and quantification using mass spectrometry analysis. Brief Funct Genomic Proteomic, 2009. 8:90-103.

Mitra, SK, Clouse, SD, and Goshe, MB. Enrichment and preparation of plasma membrane proteins from Arabidopsis thaliana for global proteomic analysis using liquid chromatography-tandem mass spectrometry. Methods Mol Biol, 2009. 564:341-355.

Clouse, SD, Goshe, MB, Huber, SC, and Li, J, Functional Analysis and Phosphorylation Site Mapping of Leucine-Rich Repeat Receptor-Like Kinases., in Plant Proteomics: Technologies, Strategies and Applications., G.K. Agrawal and R. Rakwal, Editors. 2008, John Wiley & Sons. p. 469-484.

Clouse, SD. The molecular intersection of brassinosteroid-regulated growth and flowering in Arabidopsis. Proc Natl Acad Sci U S A, 2008. 105:7345-7346.

Clouse, SD, Brassinosteroid Signaling, in Plant Cell Monographs, L. Bogre and G. Beemster, Editors. 2008, Springer-Verlag Berlin: Heidelberger Platz 3, D-14197 Berlin, Germany. p. 179-197.

He, K, Gou, X, Powell, RA, Yang, H, Yuan, T, Guo, Z, and Li, J. Receptor-like protein kinases, BAK1 and BKK1, regulate a light-dependent cell-death control pathway. Plant Signal Behav, 2008. 3:813-815.

Goshe, MB. Characterizing phosphoproteins and phosphoproteomes using mass spectrometry. Brief Funct Genomic Proteomic, 2006. 4:363-376.