Arabidopsis 2010: Functional Analysis and Phosphorylation Site Mapping of Leucine-Rich Repeat Receptor-Like Kinases in Arabidopsis

National Science Foundation (MCB)0419819

in vitro phosphorylation site database

We developed a high-throughput protocol for analyzing LRR RLK in vitro autophosphorylation sites using our Premier Q-ToF mass spectrometer functioning in both data-dependent acquisition (LC/MS/MS) and data-independent (LC/MSE) modes. Analyses with both unenriched and IMAC-enriched peptide samples for over 100 LRR RLKs, with four injections per LRR RLK, were performed. We are currently compiling and manually assessing the data from these measurements and the first set of manually verified data is accessible by clicking on the link below. The database is being continually updated with additional sites. Once within the database, clicking on an individual peptide sequence will pull up the MS spectrum and Mascot analysis for that peptide. When several Ser and Thr residues appear within a single paranthesis, Mascot has assigned a specific residue as phosphorylated, but manual inspection suggests that other residues within the paranthesis are equally likely to be phosphorylated, and thus the true phosphorylation site is still ambiguous for that particular peptide. Confirmed, unambigous identifications have an amino acid residue number assigned.

ENTER THE IN VITRO PHOSPHORYLATION SITE DATABASE BY CLICKING HERE