Honors Requirements - Fall 2009

1.         Attend two research seminar and write [type, not hand written] a two page summary double spaced to contain the points listed below. This is open to all students but must be done by all honors students. Final day for accepting the reports will be the last day of class. Two points awarded for each report. The report should address the following questions:

            a.         Who spoke

            b.         Where was the person from

            c.         What was the title of the seminar talk

            d.         What were the main points of the seminar talk

2.         Valid seminar series:

            a.         Biochemistry seminar - Thursdays @ 4 pm in 128 Polk - biochem.ncsu.edu/seminar.php

            b.         Protein journal club - Fridays @ 12:15 pm in 128 Polk

            c.         Genetics seminar - Mondays at 1:30 p.m. in 3503 Gardner Hall http://www.cals.ncsu.edu/genetics/seminar.html

            d.         Microbiology seminar - Thursdays @ 11:00 am in the Stevens Room [3503 Gardner Hall] www.microbiology.ncsu.edu/news/seminars.html [Double check this as the spring seminar list is not showing.]

            e.         PB&J - plant biology seminar - Tuesdays @ 4:00 pm - location 101 David Clark Labs http://cals.ncsu.edu/plantbiology/seminar.html

            f.         Others? - check with the instructor

3.         Enzyme report: You will be assigned one of these enzymes to investigate:

A.        Hexokinase

B.        Glucose-6-phosphate isomerase

C.        Phosphofructokinase-1

D.        Aldolase

E.        Triose phosphate isomerase

F.        Glyceraldehyde-3-phosphate dehydrogenase

G.        Phosphoglycerate kinase

H.        Phosphoglycerate mutase

I.         Enolase

J.         Pyruvate kinase

K.        Pyruvate dehydrogenase

L.        Pyruvate decarboxylase

M.       Pyruvate carboxylase

N.        Alcohol dehydrogenase

O.        Lactate dehydrogenase

P.        Citrate synthase

Q.        Aconitase

R.        Isocitrate dehydrogenase

S.        α-ketoglutarate dehydrogenase

T.        Succinyl-CoA synthetase

U.        Fumarase

V.        Malate dehydrogenase

W.       Phosphoenolpyruvate carboxykinase

X.        Fructose 1,6 bisphosphatase

Y.        Phosphofructokinase-2

Z.        Glycerol-3-phosphate dehydrogenase

AA.     Glycerol kinase

BB.     Glycogen synthase

CC.     Glycogen phosphorylase

DD.     Isocitrate lyase

EE.      Malate synthase

FF.      NADH-ubiquinone oxidoreductase

GG.     Ubiquinol-cytochrome c oxidoreductase

HH.     Cytochrome C oxidase

II.        ATP synthase

For your enzyme, prepare a written report in electronic form to be shared with the rest of the class containing the following information. You will be called upon during class when we get to your enzyme. [This will happen during the last third of the semester.]

•          EC # [enzyme commission number] 

•          Major commercial source [what organism is it isolated from] 

•          Molecular weight 

•          Number of subunits 

•          Km for substrates 

•          Original discoverer and discovery date

[One good source of information is the Sigma catalog. Another is Methods in Enzymology. If you find more that one organism for your enzyme, report the information which most closely matches the human enzyme.]

Enzyme assignments: 

000212025      Z

000743913      A

000747335      E

000748587      F

000763593      G

000771265      Z

000772890      DD

000775027      K

000776236      Q

000776863      Y

000776935      B

000778670      CC

000779132      R

000780423      D

000790253      EE

000793340      D

000793673      FF

000793676      HH

000795763      FF

000795861      Y

000798563      V

000804584      GG

000804898      BB

000805778      W

000806158      H

000806201      U

000806805      B

000806807      C

000806914      U

000807688      DD

000808436      C

000808445      E

000808575      L

000808981      I

000809389      HH

000809623      R

000809626      S

000809630      T

000809637      W

000810957      A

000811162      G

000812269      K

000812359      N

000812458      V

000812891      EE

000812928      GG

000813306      F

000813408      L

000813772      AA

000813778      BB

000814184      M

000814465      AA

000814875      H

000815637      II

000820217      T

000821007      J

000823067      O

000827140      X

000827945      N

000828304      P

000829240      X

000829454      I

000829465      J

000900653      Q

000902904      M

000905897      S

000911144      O

000927063      P

000933247      CC