Horowitz Laboratory

    Department of Molecular Biomedical Sciences

 

Research in the Horowitz laboratory focuses on the regulation of mammalian cell transcription and proliferation.  In the last several years we have focused the majority of our attention on understanding the roles of (i) the Sp-family of transcription factors and (ii) Nkx3.1, a homeodomain-containing transcription factor and tumor-suppressor gene, in the regulation of proliferation, differentiation, and animal development.

 

The laboratory employs a number of different approaches to studying Sp proteins ad Nkx3.1.  For example, we have reported a series of studies focusing on regulatory mechanisms governing the subcellular localization of Sp-family members and Nkx3.1. These studies include the use of indirect immunofluorescence in fixed cells, and GFP-fusion proteins expressed in live cells that are monitored using time-lapse confocal microscopy. 

 

     

More recently we have generated a series of transgenic and "knockout" mouse lines in which the activities of particular genes have been accentuated or inactivated.  These studies have led to the realization that the genes under study govern fundamental cellular processes, such as decisions leading to stem cell differentiation.

    

These clues from mouse models have encouraged us to extend our studies to events in early embryogenesis.  To do so we have taken advantage of yet another animal model system, that of the zebrafish Danio rerio, to explore the activities of our genes at the earliest stages of metazoan development.  

                            

For details regarding ongoing projects as well as a list of recent publications from the laboratory follow this link.

 

This page was last updated on 07/16/08.

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