Course information for Introduction to Applied Bioprocessing
Course #	BEC(MB)180
Instructor	Jason Andrus, Ph.D. 4015 Gardner Hall Addition North Carolina State University Raleigh, NC 27695	email: andrusja@meredith.edu
	Dr. José Manuel Bruno-Bárcena 1552A Gardner Hall Addition North Carolina State University Raleigh, NC 27695	Phone: 919-513-1495 Fax: 919-513-8235 email: jbbarcen@ncsu.edu web site: http://www4.ncsu.edu/~jbbarcen
Prerequisites	High school Biology and Chemistry
Class Hours Office Hours	Lecture - Laboratory– Friday, July 06^th – July 31^st, from 9 am to 3:30 pm Tuesday, 1500-1530 Monday
Credit Hours	2
Restrictions	Who should apply? Applicants should have a strong interest in biotechnology and/or science. Applicants should have completed the necessary prerequisite high school courses.
Course Website	http://www.cifr.ncsu.edu/scibls/
Delivery Format	This is a Summer class. Students are required to attend daily lectures and laboratories during the weeks the course is taught.
Course Description	The course introduces students to basic biomanufacturing tools and experiences, coupled with data analysis, interpretation, and presentation. The course provides a hands-on learning experience that challenges students to enhance existing skills, learn new information, and apply learning to understand biomanufacturing fundamentals. The educational environment will emphasize team-based decisions, collaborations and consideration of multiple perspectives. The course culminates with students cloning and growing cells, expressing, clarifying, purifying, formulating and packaging vials of a recombinant protein. Students successfully completing this experience will understand the fundamentals of biomanufacturing, empowering them to intelligently evaluate biotechnology career options. Through this course, students will apply skills and perform operations that are relevant to the biomanufacturing industries. Transportation will be provided for field trips to local biomanufacturing facilities.
Technology Requirements	In order to complete the course, all students will be required to have access to an active internet connection. If you do not have Adobe Acrobat Reader installed on your computer, you will need to go to the following web site and follow the instructions to download a free version. http://www.adobe.com/products/acrobat/readstep2.html
Text Requirements	All required reading material is contained within the course or is available through a World Wide Web link provided within the course content. At present, all laboratory reading materials will be provided. The class links page is also available as a source of the following references for the module: 1. Shuler, M.L., Kargi, F., editors 2002. Bioprocess Engineering: Basic Concepts. Prentice-Hall of India (ISBN0130819085) $127.00 2. Harley-Prescott 2002. Laboratory Exercises in Microbiology, Fifth Edition. The McGraw-Hill Companies. (ISBN0072333456) $70.63 These references will also be available in the Reserve Room of the D. H. Hill Library.
Learning Outcomes	At the end of this course, you should be able to: Start, streak, bank, sample, analyze and store cultures Quantify cell populations via microscopy, optical density and plating Safely perform the basic biomanufacturing steps of transformation, cellular growth, induction, cell harvest, homogenization, clarification, column chromatography, buffer exchange, formulation and vialing. Determine and analyze results of laboratory experiments.

Lecture & Laboratory Outlines by Topical Areas	Week 1 Read Chapter 2, An Overview of Biological Basics, Section 2.1 (pp. 11-25), and Sections 2.3-2.4 (pp. 46-54) Prior to the Unit 1 Lab, review the section of your lab manual entitled ORIENTATION TO THE LABORATORY: RULES OF CONDUCT AND GENERAL SAFETY. Monday [Orientation] [Campus Tour] Safety Partner Selection Waste Disposal Review & Preview Tuesday Quiz Measures & Exercises Special Project Aseptic Techniques Wednesday Read Exercise 13 Lab Manual Quiz Luria plating Contamination Plates Media Prep and background Review & Preview Review & Preview Thursday Read Exercise 19 Lab Manual Quiz Plate inspection Serial dilutions Viability Plating Dry Weight vs Wet Weight Review & Preview Friday Quiz Dry Weight vs Wet Weight Plate Counting Weekend Homework Review & Preview SCIBLS Poster Training Week 2 Monday Quiz Contamination plate inspection Notebook Review Spectroscopy Measurement Concentration Linearity Review & Preview Tuesday Read Exercise 1 Lab Manual Quiz /Dry weight Analysis Microscopy Pre-made Hemocytometer Sizing Wet Mount Review and Preview Wednesday Quiz/ Homework Presentation Special Project How to give a presentation Review & Preview SCIBLS – CALS Career Services Thursday Quiz Biomanufacturing Overview Two Weeks in Review – Student Presentation Review & Preview Company Tour Friday Read the following: Chapter 4, How Cells Work, Sections 4.1 – 4.5 (pp. 105-119) No Quiz Cell banking Dairy Processing Overview Genetics Background Weekend Homework Review & Preview	Week 3 Chapter 6, How Cells Grow, read the following: Sections 6.1 - 6.2.2  (pp. 155-168) Monday Read Exercise 45 Lab Manual Quiz Shake Flask OD Growth Cell bank tests Dairy Pilot Plant Tour GFP Plasmid Purification Review & Preview Tuesday Read Biotechnology Explorer™ pGLO™ Bacterial Transformation Kit Quiz Cell Bank tests GFP Transformation Reactor Design Special Project & Presentation Review & Preview Wednesday Read Chapter 8, How Cellular Information Is Altered, Sections 8.1 – 8.6 (pp. 219-241) Quiz GFP Flask Growth GFP % Transformation Cell Bank -Student Presentation GFP Expression Notebook Review Review & Preview Thursday Quiz Protein Quantification GFP % Transformation Cell Recovery Centrifugation Review & Preview Friday Read Biotechnology Explorer™ pGLO™ Bacterial Transformation Kit Quiz Poster Project GFP cell disruption & viability Homogenization GFP quantification Review & Preview Week 4 Monday Quiz Lysis viability reduction Poster work GFP chromatography Chromatography Review & Preview Tuesday Quiz GFP Filtration Tangential Flow Filtration Formulation and Vialing GFP Vialing Review & Preview Wednesday Quiz Analytical results Poster Work Course Review BTEC Programs Course Evaluation Review & Preview Thursday Test Notebook Review [Poster – Deadline] [Poster Presentation Practice] [Final Thoughts] Friday [Poster Setup] Poster Session [Lunch] [Closing Comments] Items in brackets [ ] are not part of the official course contact hours
Course Grading	Weekly lab quizzes -10 questions/15 minutes (35%) Weekly lab skills demonstrations (15%) Lab notebook, organism identification, and lab reports are due on the second to last day of class (25%) Final Exam (25%) – The final exam will be held on the second to last day of class. Attendance at ALL laboratories is mandatory and unexcused absence from lab will result in failure of the course. Lecture attendance is also required and non-attendance will result in a reduction of 10 points in the final grade. Students taking the course on a credit-only basis will be required to read each lesson and complete the quizzes plus the exams, and to earn an S grade, the overall score must be at least 69.0.

Grading Scale

A+ A A- B+ B B- C+ C C- D+ D D- F

= = = = = = = = = = = = =

97.0-100% 92.0-96.9% 89.0-91.9% 86.0-88.9% 82.0-85.9% 79.0-81.9 % 76.0-78.9% 72.0-75.9% 69.0-71.9% 66.0-68.9% 62.0-65.9% 59.0-61.9% < 59.0%

Policy on Late Assignments and Incomplete Grades

Assignments are due on or before the time and date indicated on the assignments. Due dates can be extended for students with valid reasons as defined by the NCSU Attendance policy at: www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.3.php.

In cases where the conflict can be anticipated, prior arrangements must be made with the instructor to receive an extension. In cases of illness or family emergency, the student may be required to present documentation or other proof to receive an extension. Late assignments without a valid excuse will not be accepted and will receive a score of zero. The university policy regarding incomplete grades (IN) applies to this course. See http://ncsu.edu/policies/academic_affairs/grades_undergrad/REG02.50.3.php for instructor’s policy on IN grades.

Academic Integrity Statement

It is expected that each student will complete his/her own homework, quizzes, and exams with academic integrity. Students shall follow the NCSU Code of Student Conduct (http://www.ncsu.edu/policies/student_services/student_discipline/POL11.35.1.php)

In addition, your signature on any test or assignment means that you neither gave nor received unauthorized aid. In other words, your signature on to-be-graded work in this course communicates an understanding of, and adherence to, the University Honor Pledge: ”I have neither given nor received unauthorized aid on this test or assignment.”

Attendance Policy

Students are expected to attend class and attendance will be taken. If there is a need to miss class, notify the instructor prior to the class. It is the student’s responsibility to obtain assignments and information for any missed classes. For NCSU attendance regulations, refer to the academic policy and regulations website at:

http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.3.php

Attendance at ALL laboratories is mandatory and unexcused absence from lab will result in failure of the course. Lecture attendance is also required and non-attendance will result in a reduction of 10 points in the final grade.

Laboratory Safety

Each student is expected to observe proper laboratory procedures as outlined in the class instructions for each laboratory period and in the Lab Safety Plan to be presented at the first laboratory meeting.

Students with Disability Policy

Reasonable accommodations will be made for students with verifiable disabilities. In order to take advantage of available accommodations, students must register with Disability Services for Students at 1900 Student Health Center, Campus Box 7509, 515-7653. http://www.ncsu.edu/dso/

Students with disabilities should contact the instructor for any additional assistance. Federal law mandates that the faculty provide reasonable accommodations to students with disabilities. (See NC State’s Academic Regulation for providing accommodations for students with disabilities.) (http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.1.php)