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Course
information for
Fundamentals of Microbial Cell Culture |
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Course
# |
MB(BEC)
320 |
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Instructor |
José M. Bruno-Bárcena 1552A Gardner Hall Addition North Carolina State University Raleigh, NC
27695 |
Phone: 919-513-1495
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Driss Elhanafi 850 Oval Drive,
Suite 195 Raleigh,
NC 27695-7928 |
Phone: 919-513-8236 Fax:
919-513-8235 email: driss_elhanafi@@ncsu.edu |
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Location |
Centennial Campus -- BTEC Building – Rooms #119 & #105. Centennial Campus OBTAIN
DIRECTIONS HERE |
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Prerequisites/ |
BIO 181 or BIO 183 or ZO 161 |
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Credit
Hours |
2 |
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Date |
From January 11 to March 1st |
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Class
Hours |
Lecture - Tuesdays, 12:50-14:40; Laboratory – Thursdays, ·
Section MB(BEC) 320L 202 Wednesday, ·
Section MB(BEC) 320L 201 |
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Office
Hours |
Tuesdays, 14:40-15:30 |
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Restrictions
& Notes |
Students who have completed MB 352 may not take
this course for credit Electronic devices are required to be
turned off in the classroom |
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Course
Website |
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Delivery
Format |
This
is a half-semester class. Students are required to attend weekly lectures and
laboratories during the weeks the course is taught. |
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Course
Description |
This
introductory course addresses fundamental cell biology concepts and enables
students to gain an understanding of the basic principles of microbiology,
culture preparation, physiology and genetics of microbial cell cultures. The
lab portion of the course provides students with practical experience in
basic laboratory and culture techniques. |
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Technology
Requirements |
In
order to complete the course, all students will be required to have access to
an active internet connection. If you
do not have Adobe Acrobat Reader installed on your computer, you will need to
go to the following web site and follow the instructions to download a free
version. |
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Text
Requirements |
All required
reading material is contained within the course or is available through a
World Wide Web link provided within the course content. The class links page is also available as a
source of the following references for the course: 1. Shuler, M.L.,
Kargi, F., editors 2002. Bioprocess Engineering: Basic Concepts.
Prentice-Hall of 2. Harley-Prescott
2002. Laboratory Exercises in Microbiology, Fifth Edition. The McGraw-Hill
Companies. (ISBN0072333456) |
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Course
Objectives |
At the end of this course, you should be able to:
v explain key concepts and principles of cell biology and microbiology,
including cell morphology, taxonomy, nutrition and growth; culture
collections and gene banks; metabolic pathways; and cell information storage
and the basics of genetic engineering; v demonstrate basic laboratory and cell culture techniques while
observing standard safety practices; and v determine and analyze results of laboratory experiments. |
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Lecture
Outlines by Topical Areas |
1.
Biological basics overview 2.
Culture Collections and Gene Banks 3.
How the Cells Grow 4.
Stoichiometry of cells and product formation 5.
Introduction to Bio-products 6.
Major metabolic pathways 7.
Cell information storage and alternations 8.
Safety in Biotechnology |
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Laboratory
Topical Areas |
1. Lab safety (equipment & policies) 2. Luria plate preparation and incubation 3. Aseptic techniques 4. Contaminant review 5. Cell concentration determination 6. Antibiotic plates 7. Create 3 working cell banks (0%, 5% & 15%
glycerol) 8. API identification 9. Cell bank viability |
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Course
Grading Structure |
Students taking the course on a non-credit basis
will be required to read each lesson and complete the quizzes plus the exams.
Students taking the course on a credit-only basis will be required to
read each lesson and complete the quizzes plus the exams, and to earn a CR
grade, the overall score must be at least 69.0. |
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Grading
Scale |
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Online Class Evaluations |
Online class
evaluations will be available for students to complete during the last week of
class. Students will receive an email message directing them to a website
where they can login using their Unity ID and complete evaluations. All evaluations are confidential;
instructors will never know how any one student responded to any question, and
students will never know the ratings for any particular instructors. Evaluation
website: https://classeval.ncsu.edu
Student help desk: classeval@ncsu.edu
More information about ClassEval: http://www2.acs.ncsu.edu/UPA/classeval/ |
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Academic
Integrity Statement |
It is expected that each student will complete
his/her own homework, quizzes, and exams with academic integrity. Students shall
follow: ·
NCSU Code of
Student Conduct
(http://www.ncsu.edu/policies/student_services/student_discipline/POL11.35.1.php
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University attendance policy (http://www.ncsu.edu/policies/academic_affairs/pols_regs/REG205.00.4.php) |
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Attendance
Policy |
Students are expected to attend class and
attendance will be taken. If there is a need to miss class, notify the instructor
prior to the class. It is the student’s responsibility to obtain assignments
and information for any missed classes. For NCSU attendance regulations,
refer to the academic policy and regulations website at: http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.3.php |
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Students
with Disability Policy |
Reasonable accommodations will be made for
students with verifiable disabilities. In order to take advantage of
available accommodations, students must register with Disability Services for
Students at 1900 Students with disabilities should contact the
instructor for any additional assistance. Federal law mandates that the faculty
provide reasonable accommodations to students with disabilities. (See NC State’s Academic
Regulation for providing accommodations for students with disabilities.) (http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.1.php) |
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