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Course information for
Fundamentals
of Microbial Cell Biotransformations |
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Course # |
MB 420/520 |
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Semester |
Fall 2011 |
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Instructor |
José Manuel Bruno-Bárcena 4554 Thomas Hall Raleigh, NC 27695 |
Phone: 919-513-1495 Fax: 919-515-7867 email: jbbarcen@ncsu.edu Web Site: http://www4.ncsu.edu/~jbbarcen |
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Requisite |
Pre-requisite MB 352 |
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Credit Hours |
2 |
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Restrictions |
Students who have completed MB 420 may not take MB 520 for credit. Electronic
devices
are required to be turned off in the classroom. |
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GEP
Status |
None |
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Location |
Room 4508 Thomas Hall |
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Date |
October 11th - December 15th |
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Class Hours |
Lecture -
Tuesdays, 12:50 PM -2:40 PM Laboratory - Time 520 PM- 10:15 PM · Wednesdays, Section MB 420L/520L Time 1250 PM- 550 PM · Thursdays, Section MB 420L/520L Room
# Small scale Fermentation Lab Thomas
Hall Building |
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Office Hours |
Tuesday, |
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Course Website |
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Delivery Format |
This is a half-semester class.
Students are required to attend weekly lectures and laboratories during the
weeks the course is taught. |
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Course Description |
This
is a half-semester course. Basic microbial cell culture theory and practice:
cell physiology, mass balances, and metabolic control as seen in a dynamic
bioreactor process to be scalable, consistent, and robust. The lab portion of
the course provides students with hands-on experience in culture techniques
using bioreactors. |
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Technology
Requirements |
In order to complete the course, all
students will be required to have access to an active internet
connection. If you do not have Adobe
Acrobat Reader installed on your computer, you will need to go to the
following web site and follow the instructions to download a free version. |
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Course
Structure |
This course will consist of two blocks of Lecture
and Laboratory. Each of the blocks will cover theory and practice of
fermentation at bench scale. After completion of each laboratory experience students
will be required to submit written reports describing objectives of the
laboratory exercise, detailed experimental procedures, and results and discussion
of the experiment. During the course of the semester students will be
regularly tested on their understanding of the theoretical portion of the
laboratory (text book reading assignments and additional reading assignments
provided by instructor) in form of quizzes (35% of total grade). Each student will be evaluated on their laboratory
safety, laboratory skills, and individual documentation skills (25% of total
grade). At the end of the course students will
complete a final exam that will cover all the topics discussed during the
course (40% of total grade). Students taking MB 520
will have the additional requirement of a major term paper. The subject of
this final paper will be to search and select from the patent database an
experimental upstream approach to produce one active pharmaceutical
ingredient (API) using cells (GMO or non GMO). The paper should be written
following the ASM journal instruction (http://jb.asm.org/misc/ifora.shtml) for authors. It
should contain at least five written pages, font 12, single paragraph. The
references pages will not be counted as the written pages. The graduate students will be graded as
described below. |
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Text Requirements |
All
required reading material is contained within the module or is available
through a World Wide Web link provided within the module content. At present,
all laboratory reading materials will be provided. The class links page is
also available as a source of the following references for the module: 1.
Shuler, M.L., Kargi, F., editors 2002. Bioprocess
Engineering: Basic Concepts. Prentice-Hall of This
reference will also be available in the Reserve Room of the D. H. Hill
.Library. |
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Learning Outcomes |
At the
end of this course, students will:
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Lecture Outlines by
Topical Areas |
Week 1. History of traditional and modern
biotechnology. Pure culture philosophy– Week 2. Cell Nutrition – Reading assignment provided by the
instructor. Quiz
#2 Week 3. Microbial cell culture and selecting the
cultivation system – Reading
assignment provided by the instructor. Quiz #3 Week 4. Elemental composition and stoichiometry of cells – Reading
assignment provided by the instructor. Quiz #4 Week 5. Microbial growth and production rate – Week 6. Microbial physiology and metabolic
control: Adaptability of cells – Reading
assignment provided by the instructor. Quiz #6 Week 7. Introduction to process instrumentation,
monitoring and supervision – Reading
assignment provided by the instructor. Quiz #7 |
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Laboratory Topical
Areas |
Week 1. Lab safety
(equipment & policies). A review of the aseptic and analytical techniques.
Week 2. Continuous
reactor cultures. Sampling and storage.
Week 3. Transitory
pulse feed. Glucose repression or Crabtree effect. Week 4. Setting
dilution rate. Substrate, product, and biomass analysis. Week 5. Calculation of
maximum specific growth rate by the wash-out and by unrestricted growth
methods. Distribute the stored data of the process from the computer. Week 6. TFF cell
harvest and UF/DF step.
Reading assignment provided by the instructor Quiz #5 Week 7. Reactor
cleaning, Reactor and probe preparation and Control unit set-up for operation. Reading assignment provided by the
instructor Quiz #6 |
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Course Grading |
For Students Taking MB 520
Attendance at ALL laboratories is mandatory and unexcused absence from lab will
result in failure of the course. Lecture attendance is also required and
non-attendance will result in a reduction of 10 points in the final grade. Students are not allowed to take
this course for "credit only". In order to receive recognition for
an audit, graduate students are required to complete all assignments and earn
a grade of C- or better. Conversion from letter grading to audit grading is
subject to university deadlines. Refer to the Registration and Records
calendar for deadlines related to grading. For more details, refer to |
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Grading Scale |
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Late Assignments |
Late assignments without a valid
excuse will not be accepted and will receive a score of zero. |
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Incomplete Grades |
Incomplete as a course grade will be awarded only for work not
completed during the course due to conditions deemed by the instructor to be
beyond the reasonable control of the student. For undergraduate students, unless an extended deadline is authorized
by the instructor or department, an unfinished incomplete grade will
automatically change to an F after either (a) the end of the next regular
semester in which the student is enrolled (not including summer sessions), or
(b) the end of 12 months if the student is not enrolled, whichever is
shorter. Incompletes that change to F will count as an attempted course on
transcripts. The University policy on incomplete grades is located at http://www.ncsu.edu/policies/academic_affairs/grades_undergrad/REG02.50.3.php For graduate students, if an extended deadline is not authorized by
the Graduate School, an unfinished incomplete grade will automatically change
to an F after either (a) the end of the next regular semester in which the
student is enrolled (not including summer sessions) or (b) by the end of 12
months if the student is not enrolled, whichever is shorter. Incompletes that
change to F will count as an attempted course on transcripts. The burden of
fulfilling an incomplete grade is the responsibility of the student. The
University policy on incomplete grades is located at http://www.ncsu.edu/policies/academic_affairs/grades_undergrad/REG02.50.3.php Additional
information relative to incomplete grades for graduate students can be found
in the Graduate Administrative Handbook in Section 3.18.F at: http://www.fis.ncsu.edu/grad_publicns/handbook/. |
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Academic Integrity
Statement |
It is
expected that each student will complete his/her own homework, quizzes, and
exams with academic integrity. Students shall follow the NCSU Code of
Student Conduct
(http://www.ncsu.edu/policies/student_services/student_discipline/POL11.35.1.php) In
addition, your signature on any test or assignment means that you neither
gave nor received unauthorized aid. In other words, your signature on to-be-graded
work in this course communicates an understanding of, and adherence to, the
University Honor Pledge: ”I have neither given nor received unauthorized aid
on this test or assignment.” |
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Attendance Policy |
Students
are expected to attend class and attendance will be taken. If there is a need
to miss class, notify the instructor prior to the class. It is the student’s
responsibility to obtain assignments and information for any missed classes.
For NCSU attendance regulations, refer to the academic policy and regulations
website at: http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.3.php Attendance
at ALL laboratories is mandatory and unexcused absence from lab will result
in failure of the course. Lecture attendance is also required and
non-attendance will result in a reduction of 10 points per missed lecture in
the final grade. |
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Laboratory Safety |
Each student is expected to observe
proper laboratory procedures as outlined in the class instructions for each
laboratory period and in the Lab Safety Plan to be presented at the first
laboratory meeting. |
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Students with
Disability Policy |
Reasonable
accommodations will be made for students with verifiable disabilities. In
order to take advantage of available accommodations, students must register
with Disability Services for Students ( http://www.ncsu.edu/dso/ ) at 1900 ( http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.1.php ) |
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Anti-Discrimination Statement |
NC State University provides
equality of opportunity in education and employment for all students and
employees. Accordingly, NC State affirms its commitment to maintain a work
environment for all employees and an academic environment for all students
that is free from all forms of discrimination. Discrimination based on race,
color, religion, creed, sex, national origin, age, disability, veteran
status, or sexual orientation is a violation of state and federal law and/or
NC State University policy and will not be tolerated. Harassment of any
person (either in the form of quid pro quo or creation of a hostile
environment) based on race, color, religion, creed, sex, national origin,
age, disability, veteran status, or sexual orientation also is a violation of
state and federal law and/or NC State University policy and will not be
tolerated. Retaliation against any person who complains about discrimination
is also prohibited. NC State’s policies and regulations covering
discrimination, harassment, and retaliation may be accessed at http://www.ncsu.edu/policies/campus_environ or http://www.ncsu.edu/equal_op. Any person who
feels that he or she has been the subject of prohibited discrimination,
harassment, or retaliation should contact the Office for Equal Opportunity
(OEO) at 515-3148.” |
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