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Course
information for Fundamentals of Microbial Cell Biotransformations |
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Course
# |
MB(BEC)
420 |
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Instructors |
José
Manuel Bruno-Bárcena 1552A
Gardner Hall Addition Raleigh, NC 27695 |
Phone:
919-513-1495 |
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Matthew Evans 850 Oval Drive,
Suite 195 Raleigh,
NC 27695-7928 |
Phone:
919-513-8094 Fax:
919-513-8235 email: mrevans@@ncsu.edu |
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Prerequisites |
BEC(MB) 320, or MB 352 |
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Credit
Hours |
2 |
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Restrictions |
Students who have completed BIT (CHE) 463
may not take this course for credit. Electronic
devices
are required to be turned off in the classroom |
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LOCATION |
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Date |
From October 14th to December 4th |
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Class
Hours |
Lecture - Tuesdays, 12:50-14:40; Laboratory - Time 520 PM- 10:15 PM · Wednesdays, Section MB 420L 205
& BEC 420L 202 Time 1250 PM- 550
PM · Thursdays, Section
MB 420L 206 & BEC 420L 201 Room
#110 Small scale Fermentation Lab BTEC Building |
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Office
Hours |
Tuesday, 14:50-15:30 |
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Course
Website |
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Delivery
Format |
This
is a half-semester class. Students are required to attend weekly lectures and
laboratories during the weeks the course is taught. |
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Course
Description |
Basic
microbial cell culture theory and practice: cell physiology, mass balances,
and metabolic control as seen in a dynamic bioreactor culture. The biological
understanding, mathematical models, and engineering controls that enable a
bioreactor process to be scalable, consistent, and robust. The lab portion of
the course provides students with hands-on experience in culture techniques
using bioreactors. |
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Technology
Requirements |
In
order to complete the course, all students will be required to have access to
an active internet connection. If you do not have Adobe Acrobat Reader
installed on your computer, you will need to go to the following web site and
follow the instructions to download a free version. |
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Text
Requirements |
All required
reading material is contained within the course or is available through a
World Wide Web link provided within the course content. At present, all
laboratory reading materials will be provided. The class links page is also
available as a source of the following references for the course: 1. Shuler, M.L.,
Kargi, F., editors 2002. Bioprocess Engineering: Basic Concepts.
Prentice-Hall of This reference will
also be available in the Reserve Room of the D. H. Hill .Library. |
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Learning
Outcomes |
At the end of this course, you should be able to:
v explain key fundamental biotechnology concepts and will be able to
understand and comprehend culturing processes used in the traditional and in
the modern biotechnology; v demonstrate laboratory and cell culture techniques using small scale
bioreactors while observing standard safety practices; and v determine and analyze results of laboratory experiments as well as be
aware of the importance of the interdisciplinary effort required for product
development. |
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Lecture
Outlines by Topical Areas |
1.
History of traditional and modern biotechnology. Pure culture philosophy 2.
Cell Nutrition 3.
Elemental composition and stoichiometry of cells 4.
Microbial growth and production rate 5. Microbial cell culture
and selecting the cultivation system 6. Microbial physiology and metabolic control: Adaptability of cells 7.
Introduction to process instrumentation, monitoring
and supervision 8.
An
overview of cGMP requirements |
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Laboratory
Topical Areas |
1. Lab safety (equipment & policies). A review of the aseptic and analytical techniques 2. Continuous reactor cultures. Sampling and
storage. 3. Transitory pulse feed. Glucose repression or Crabtree
effect 4. Setting dilution rate. Substrate, product, and
biomass analysis
5. Calculation of
maximum specific growth rate by the wash-out and by unrestricted growth
methods. Distribute the stored data of the process from the computer. 6. TFF cell harvest
and UF/DF step 7. Reactor cleaning, Reactor and probe preparation and Control unit
set-up for operation Before the Final Test you should make available
the complete notebook rendering data analysis, graphs and conclusions. |
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Course
Grading |
Students taking the course on a non-credit basis
will be required to read each lesson and complete the quizzes plus the exams.
Students taking the course on a credit-only basis
will be required to read each lesson and complete the quizzes plus the exams,
and to earn a CR grade, the overall score must be at least 69.0. |
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Grading
Scale |
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Policy
on Late Assignments and Incomplete Grades |
Assignments are due on or before the time and
date indicated on the assignments. Due dates can be extended for students
with valid reasons as defined by the NCSU Attendance policy at: www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.3.php.
In cases where the conflict can be anticipated, prior arrangements must be
made with the instructor to receive an extension. In cases of illness or
family emergency, the student may be required to present documentation or
other proof to receive an extension. Late assignments without a valid excuse
will not be accepted and will receive a score of zero. The university policy
regarding incomplete grades (IN) applies to this course. See http://ncsu.edu/policies/academic_affairs/grades_undergrad/REG02.50.3.php
for instructor’s policy on IN grades. |
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Academic
Integrity Statement |
It is expected that each student will complete
his/her own homework, quizzes, and exams with academic integrity. Students shall
follow the NCSU Code of Student Conduct
(http://www.ncsu.edu/policies/student_services/student_discipline/POL11.35.1.php) In addition, your signature on any test or
assignment means that you neither gave nor received unauthorized aid. In
other words, your signature on to-be-graded work in this course communicates
an understanding of, and adherence to, the University Honor Pledge:”I have
neither given nor received unauthorized aid on this test or assignment” |
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Attendance
Policy |
Students are expected to attend class and
attendance will be taken. If there is a need to miss class, notify the
instructor prior to the class. It is the student’s responsibility to obtain
assignments and information for any missed classes. For NCSU attendance
regulations, refer to the academic policy and regulations website at: http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.3.php Attendance
at ALL laboratories is mandatory
and unexcused absence from lab will result in failure of the course. Lecture
attendance is also required and non-attendance will result in a reduction of
10 points in the final grade. |
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Laboratory
Safety |
Each student is expected to observe proper
laboratory procedures as outlined in the class instructions for each
laboratory period and in the Lab Safety Plan to be presented at the first
laboratory meeting. |
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Students
with Disability Policy |
Reasonable accommodations will be made for
students with verifiable disabilities. In order to take advantage of
available accommodations, students must register with Disability Services for
Students at 1900 Students with disabilities should contact the
instructor for any additional assistance. Federal law mandates that the faculty
provide reasonable accommodations to students with disabilities. (See NC State’s Academic Regulation
for providing accommodations for students with disabilities.) (http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.1.php) |
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