José Manuel Bruno Bárcena, Ph. D.

Assistant Professor of Microbiology

		Asturias Cheeses
	CONTACT INFORMATION North Carolina State University Department of Microbiology & BTEC 4510 Gardner Hall Campus Box 7615 Raleigh, NC 27695 Obtain Directions to Microbiology North Campus MAP Look for Gardner Hall Building	Office: (919) 513-1495 Laboratory: (919) 513-3834 Fax: (919) 515-7867 jbbarcen@@ncsu.edu Obtain Directions to BTEC Centennial Campus MAP Look for Research Building II

Upstream Bioprocess Development Specialist with over 12 years of experience in team building and management, academic research, design of pilot plants and process development laboratories (up to 500 liters) including analytical and process equipment identification and operation.

SUMMARY

I graduated as a Biologist at the University of Oviedo, Spain, in 1991. From 1994 to 1999, I worked at the Pilot Plant for Microbial Processes (CONICET-PROIMI) and successfully completed my Ph.D. Thesis in 1997 at the University of Tucumán, Argentina. From 1999 to 2002, I was a researcher in the Institute of Agrochemical and Food Technology (IATA) in Valencia, Spain. There, I assembled the fermentation and process development capabilities of the center, being also responsible for managing the technical operations of the laboratory, including resources and personnel management. I developed processes for pharmaceutical companies and actively promoted IATA´s capabilities to produce custom fermentations for third parties in Europe. From 2002 to 2005, at North Carolina State University, I served as a researcher studying the molecular responses of microorganisms to reactive oxygen species. In 2005, I was appointed by NC State University as a Research Assistant Professor and promoted in 2007 to Assistant Professor to serve in the Department of Microbiology and Biomanufacturing Training and Education Center. Since then, developed bioprocessing related courses and provided the expertise that led to the establishment of the process development BTEC laboratory. Presently, I manage daily operations while actively serving in the Microbiology Department, my primary appointment. My research interests include microbial physiology, process development, and optimization of cell culture methodologies. I have over 15 publications in peer-reviewed journals and books.

COURSE OFFERING

MB (BEC) 180

Introduction to Applied Bioprocessing

MB (BEC) 320

Fundamentals of Microbial Cell Culture

MB (BEC) 420

Fundamentals of Microbial Cell BioTransformations

RESEARCH INTEREST

UNESCO codes: 241402-241403-330203;

Cell physiology, Cell Metabolism, Industrial Bioprocessing and Microbiology

PROFESSIONAL AND RESEARCH EXPERIENCE:

Since Dec. 2007 Assistant Professor. NC State University (NCSU) Microbiology Department, USA. Primary appointment

Since Mar. 2006 Teaching Assistant Professor. North Carolina Bio-manufacturing Training and Education Center (NC BTEC). Associate Faculty

March 2005 to 2007 Research Assistant Professor. North Carolina State University (NCSU) Microbiology Department, USA

North Carolina Bio-manufacturing Training and Education Center (NC BTEC)

April 2002-2005 Research Associate. North Carolina State University (NCSU) Microbiology Department, USA

Conducted research in the topic: Oxidative stress affecting physiology of lactic acid bacteria.

2002-2003 Research Scientist. Consejo Superior de Investigaciones Científicas (CSIC-IATA). Spain

Conducted research in the topic: Integral automatization of biotechnological processes during microbial growth. Application of the on-line estimation of biological species and magnitudes of interest to the acquisition of concentration values through intelligent sensors. (Polytechnic University of Valencia, CSIC-IATA). Financial support by Generalitat Valenciana-Spain (GV01-364).

2000-2001 Research Scientist. Consejo Superior de Investigaciones Científicas (CSIC-IATA). Spain

Founder technical director responsible for setting up a pilot plant for the industrial production of additives, starters and diagnostic kits to be used by food industries. Financial support by European Union FEDER-Program (1FD97-0759). Currently, CSIC «spin-off» as the biotechnological company named Biópolis S.L. (http://www.biopolis.es/).

1999-2000 Postdoctoral Fellow. Consejo Superior de Investigaciones Científicas (CSIC-IATA). Spain

Conducted research in the topic: Secretion of extracellular enzymes by fungus of the genus Aspergillus. Financial support by European Union FEDER-Program (1FD97-1134-C03-03).

Conduced research in the topic: Production by biotechnological methods of enzymes used in the food technology; specifically those used in the wine production. Financial support by Spanish Program for the Scientific Cooperation with Latin America. Projects for the cooperation research.

1998-1999 Research Scientist. CONICET-PROIMI. Argentina

Conducted research in the topic: Microbial additives and microbial enzymes of technological interest. Financial support by National Council for Scientifics and Technical Research (CONICET 2851). Projects INNOVA-T, PIP Nº 0278/98.

1997-1998 Research Scientist. CONICET-PROIMI. Argentina

Conducted research in the topic: Development and Optimization of Biotechnological Process. Financial support by Argentinean National Agency for the Promotion of Scientific and Technological Research (ANPCyT), PICT 97–Nº 09-00091-01979.

Conducted research in the topic: Optimization of Biotechnological Processes. Financial support by Banco Interamericano de Desarrollo. R&D Projects (BID-PID-0304).

EDUCATION

April, 1997 Universidad Nacional de Tucumán, Tucumán, Argentina
PhD in Biology
Dissertation: Production of biomass and L(+)-lactic acid by selected strains of lactic acid bacteria.

February, 1991 Universidad de Oviedo, Asturias, Spain.

B.S. in Biology.

AWARDS AND FELLOWSHIPS:

Since 2007 Society for Industrial Microbiology

2005 Sigma Xi, North Carolina State University Chapter Scientific Research Society

Since 2005 Graduate Faculty, North Carolina State University

2004 Member of American Society for Microbiology (ASM)

1994-1996 Spanish Scholarship award (Agency for International Cooperation (AECI)) for Ph.D. studies at the University of Tucumán, Argentina

1989-1990 Spanish Scholarship award (Agency for Science and Education (MEC). Financial support for undergraduate studies

1988-1989 Scholarship University of Oviedo, Asturias, Spain. Tuition exemption for undergraduate studies

REVIEWER ACTIVITY:

Sporadic Reviewer for Food Microbiology

Since 2005 Reviewer for Applied Environmental Microbiology, Journal Bacteriology, and Applied Microbiology and Biotechnology

Since 2005 Reviewer for International Journal of Biomedical Science

PEER REVIEW JOURNAL ARTICLES:

Carroll IM, Andrus JM, Bruno-Bárcena JM, Klaenhammer TR, Hassan HM, Threadgill DS (2007) The Anti-inflammatory Properties of Lactobacillus gasseri Expressing Manganese Superoxide Dismutase (MnSOD) Using the Interleukin 10-Deficient Mouse Model of Colitis. Am J Physiol Gastrointest Liver Physiol. 293:G729-38.

E. Pico-Marco, J.L. Navarro, J.M. Bruno-Bárcena (2006) A closed loop exponential feeding law: invariance and global stability analysis. Journal of Process Control. 16:395-402

M. Andrea Azcárate-Peril, J.M. Bruno-Bárcena, Hosni M. Hassan, T.R. Klaenhammer (2006) Transcriptional and Functional Analysis of Oxalyl-CoA Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus. Appl. Environ. Microbiol. 72: 1891-1899

R. Pérez-Torrado, J.M. Bruno-Bárcena and E. Matallana (2005). Monitoring stress related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making. Appl. Environ. Microbiol. 71: 6831-6837

J.M. Bruno-Bárcena, M.A. Azcárate-Peril, Todd R. Klaenhammer and Hosni M. Hassan (2005) Marker-free Chromosomal Integration of the Manganese Superoxide Dismutase Gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri. FEMS Microbiol. Lett. 246: 91-101

J.M. Bruno-Bárcena, J.M. Andrus, S. Libby, T.R. Klaenhammer and H.M. Hassan (2004) Expression of a Heterologous Manganese Superoxide Dismutase Gene in Intestinal lactobacilli provides protection against hydrogen peroxide toxicity. Appl. Environ. Microbiol. 70: 4702-4710

J.M. Bruno-Bárcena, M.E. Lucca, F. Siñeríz and D. Ramón (2002) pH regulation of enzyme production in Aspergillus nidulans growing in aerobic carbon-limited batch fermentor. Biotechnology Letters 24: 567-572

G. Raya Tonetti, P. R. Córdoba, J.M. Bruno-Bárcena, F. Siñeríz and N. I. Perotti (1999) Fluidized bed ion exchange for improving recovery of lactic acid from fermentation. Biotechnology Techniques 13: 201-205

J.M. Bruno-Bárcena, A.L. Ragout, P.R. Córdoba and F. Siñeríz (1999) Continuous production of L(+) lactic acid by Lactobacillus casei in two-stage systems. Appl. Microbiol. Biotechnol. 51: 316-324

J.M. Bruno-Bárcena, F. Siñeríz, D. González de Llano, A. Rodríguez and J.E. Suárez (1998) Chemostat production of plantaricin c by Lactobacillus plantarum LL 441. Appl. Environ. Microbiol. 64: 3512-3514.

J.M. Bruno-Bárcena, M.A. Azcárate, A.L. Ragout, G. Font de Valdez, R.R. Raya and F. Siñeríz (1998) Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation. Biotechnology Techniques 12: 97-99

S. C. Ginés, J. M. Bruno–Bárcena, A. Ragout, G. F. de Valdez (1997) Effect of the carbon source on the fermentation balance of Lactobacillus reuteri. Microbiologie-Aliments-Nutrition. 15: 23-27

G.F. de Valdez, A.L. Ragout, J.M. Bruno-Bárcena, H. Diekmann and F. Siñeríz (1997) Shifts in the pH affect the maltose glycerol cofermentation by Lactobacillus reuteri. Biotechnology Letters 19: 645-649

BOOK CHAPTERS:

J.M. Bruno-Bárcena, E. Picó-Marco, J.L. Navarro and J. Picó (2007) Microbial Dynamic Transformations: Basic Concepts. Encyclopedia of Life Support Systems (EOLLS). UNESCO

J.M. Bruno-Bárcena and F. Siñeríz (2002) Industrial Biotechnology. Encyclopedia of Life Support Systems (EOLLS). UNESCO

J.M. Bruno-Bárcena, M.A. Azcárate-Peril and F. Siñeríz (2001) Genetically modified organisms (GMO) in food products: potential risks and the reaction of the consumer. Food Protocols, 481-484. Humana press, Totowa-New Jersey.

J.M. Bruno-Bárcena, A.L. Ragout, P.R. Córdoba and F. Siñeríz (2001) Reactor configuration for fermentation in immobilized continuous system. Food Protocols, 321-328. Humana press, Totowa-New Jersey.

J.M. Bruno-Bárcena, A.L. Ragout and F. Siñeríz (1998) Microbial physiology applied to process optimization: lactic acid bacteria. Advances in Bioprocess Engineering II. Vol. II, 97-110. Kluwer Academic Publishers, The Netherlands

PATENTS:

Bishop, P.E., T.M. Loveless, J. Olson, and J.M. Bruno-Bárcena (2007) Hydrogen Production via Nitrogenase in Tungsten-tolerant Azotobacter vinelandii. P.C. 0032.06. Serial No. 60/959,940 - Filed July 18, 2007

Hassan, H.M., J. M. Bruno-Bárcena and T.R Klaenhammer (2004) Use of Gene Replacement Technology to Construct Food-Grade Lactobacillus spp. Capable of Expressing Superoxide Dismutase and Other Antioxidant Enzymes; and its Applications Solicitude: pending. Priority Country: USA. NCSU

Navarro, J.L., J. Picó Marcó, E. Picó Marcó, J.M. Bruno-Bárcena, S. Valles (2001) On-line method and equipment for detecting, determining the evolution and quantifying microbial biomass and other substances that absorb light along the spectrum during biotechnological processes. Solicitude: European Patent Office Nº: 02751179.9-2204-ES0200355. 16/07/02. Priority Country: Europe. UPV & CSIC

Bruno-Bárcena, J.M.; Ragout, A. L, Molina, O. & Siñeríz, F. (1999) Method for the conservation of microorganisms. Solicitude: Nº 1986/450. 20/10/1999. Priority Country: Argentina. Bio-Sidus S.A. & CONICET

POSTER PRESENTATIONS:

T.M. Loveless, J. Olson, J.M. Bruno-Bárcena, P. Bishop. Biological Hydrogen Production via Nitrogenase 3 in a Tungsten-Tolerant Mutant of Azotobacter vinelandii Poster. 108th General Meeting of the American Society for Microbiology. Boston, GA, USA. Jun, 2008.

M.E. Azcárate-Peril, J.M. Bruno-Bárcena, H.M. Hassan and T.R. Klaenhammer. Oxalyl-CoA Decarboxilase and Formyl-CoA Transferase genes contribute to oxalate degradation by Lactobacillus acidophilus NCFM. 8^th Symposium on Lactic Acid Bacteria: Genetics, Metabolism and Applications. Egmond aan Zee , The Netherlands, August 28 to September 1, 2005.

E. Picó Marcó, J.M. Bruno-Bárcena. Control de bioreactors en fedbatch: Regulación de μ. Poster. XXII Jornadas de Automática, Barcelona, Spain, september, 2001

http://www.cea-ifac.es/actividades/jornadasXXII.htm

M.E. Lucca, J.M. Bruno-Bárcena, D. Ramón and F. Siñeríz. Expression of Aspergillus niger glucose oxidase with transgenic Aspergillus nidulans strains in batch fermentor. Poster. BIOLATINA. Buenos Aires, Argentina, 2000.

J.A. Romero-Pérez, J.L. Navarro-Herrero and J.M. Bruno-Bárcena. Identificación del Modelo de un Biorreactor. Poster. XXI Jornadas de Automática. F. R. Rubio (Ed.) ISBN: 84-699-3163-6. Sevilla – España, 2000.

http://www.cea-ifac.es/actividades/jornadasXXI.htm

J.M. Bruno-Bárcena, P. Córdoba, A. Ragout and F. Siñeríz. Reactores en paralelo con células inmovilizadas para la producción de ácido láctico. Poster. VIII Mexican congress of biotechnology and bioengineering . Huatulco - Oaxaca – México, September, 1999.

T. Raya, P. Córdoba, J.M. Bruno-Bárcena, F. Siñeríz and N.I. Perotti. Fluidized bed reactor for lactic acid recovering from fermentation. Poster. 98^th General Meeting of the American Society for Microbiology. Atlanta – USA. May, 1998.

T. Raya, P. Córdoba, J.M. Bruno-Bárcena, F. Siñeríz and N. I. Perotti. Lactic acid from fermentation purified in a quick downstream process using an anionic fluidized bed reactor. Poster. VII Mexican Congress of Biotechnology and Bioengineering. Mazatlán–México, 1997.

A. Ragout, J. M. Bruno-Bárcena, G. Font de Valdez, J. F. T. Spencer and F. Siñeríz. Production of cells of Lactobacillus reuteri and Saccharomyces diastaticus in mixed, continuous culture. Poster. VII Mexican Congress of Biotechnology and Bioengineering. Mazatlán – México, 1997.

C. Rey, F. Siñeríz, G. Font de Valdez, J. M. Bruno-Bárcena, A. Ragout and M. Baigorí. Differentiation between Lactobacillus fermentum and Lactobacillus reuteri strains using RAPD. Poster. 97^th General Meeting of the American Society for Microbiology. Miami Beach, Florida – USA, May, 1997.

M. A. Azcárate, J. M. Bruno-Bárcena, A. P. De Ruiz Holgado, R. R. Raya. Desarrollo de un método de extracción de ADN plasmídico para cepas de Lactobacillus. Poster. IV Congreso Latinoamericano de Microbiología e Higiene de los Alimentos. Lima – Perú, April, 1995.

A. Ragout, P. Córdoba, J. M. Bruno Bárcena, F. Siñeríz, R. Kaul and B. Mattiasson. Inert supports for lactic acid fermentation by Lactobacillus casei RS93 in upflow reactors. Poster. International symposium on immobilized cells, The Netherlands, 1995.

J. M. Bruno-Bárcena, A. Ragout, F. Siñeríz, G. Font de Valdez. Influence of the growth conditions on the morphology of L. casei strains. Poster. 7^th International Symposium on Microbial Ecology. San Paulo-BRAZIL, 1995

L. Auad, A. Azcárate, J. Breccia, S. Brizzio, J. M. Bruno-Bárcena, S. Cuozzo, C. Estévez, A. Martínez, G. Raya, L. Rubinstein, E. Schallmach, M. Taranto, M. Baigorí, G. Castro and F. Siñeríz. Diversity of thermoresistent bacteria in an effluent of sugar-cane factory of north-west of Argentina. Poster. 7^th International Symposium on Microbial Ecology. San Paulo-BRAZIL, 1995.

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