Yoder Lab: Research Interest

Photo-chemical control of gene and transgene expression in zebrafish

The zebrafish has become a powerful tool for dissecting vertebrate gene function during embryogenesis. Many systems have been developed for examining gene function in zebrafish, the most popular being transgenics for amplifying gene function and anti-sense strategies for knocking down gene function. However, only a few systems have been developed that permit spatio-temporal control of gene or transgene expression in the zebrafish embryo. More importantly, no system has been developed that is switchable, where individual genes or transgenes can be turned ON and OFF with a high degree of temporal, spatial, and quantitative control during development.

Our lab is collaborating with Alex Deiters' laboratoratory in the Department of Chemistry here at NCSU in order to develop and provide to the zebrafish community novel, switchable gene control systems for dissecting gene function in a spatio-temporal manner during zebrafish embryogenesis. The goal of our lab is to utilize these novel systems to inactivate immune-response genes during the larval stage of zebrafish development.

Related Publications:
f1000 Deiters, A., R.A. Garner RA, H. Lusic, J.M. Govan, M. Dush, N.M. Nascone-Yoder, and J.A. Yoder. 2010. Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos. J. Am. Chem. Soc. 132:15644-50. PMID: 20961123
Young, D.D., R.A Garner, J.A. Yoder and A. Deiters. 2009. Light activation of gene function in mammalian cells via ribozymes. Chem. Commun. 568-570. PMID: 19283293
Young, D.D., H. Lusic, M.O. Lively, J.A. Yoder and A. Deiters. 2008. Gene Silencing in mammalian cells with light-activated antisense agents.  ChemBioChem. 9: 2937-2940. PMID: 19021142
Deiters, A and J.A. Yoder.  2006.  Conditional transgene and gene targeting methodologies in zebrafish.  Zebrafish3: 415-429. PMID: 18377222