PROTOCOL 002: GLYCEROL STOCK OF PLASMID-CONTAINING BACTERIA
Screw-cap 1.5 mL tube
**NOTE** All procedures should be performed aseptically.
Cut a length of lab tape slightly longer than the circumference of the screw-cap tube.
Write the following information on the label:
Antibiotic resistance: Amp, Kan, Tet, etc./Strain of E. coli (DH5a, Top10F, etc).
Place label around screw-cap tube.
Transfer 850 mL from culture flask to screw-cap tube.
Add 150 mL of glycerol.
Carefully pipette solution to ensure thorough mixing.
Place in -80¡ C freezer in appropriate glycerol stock box.
Enter Rack, Box and Slot information into Plasmid Database.