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| Perturbation
of Ca2+ Homeostasis in transgenic tobacco cells (NT1) over-expressing
maize calreticulin, an ER Ca2+-storage protein Staffan Persson and John Love In order to study cellular roles of Ca2+, tools are needed to perturb Ca2+ homeostasis. The endoplasmic reticulum (ER) is an important Ca2+ store in plant cells. The retention of Ca2+ in the ER is partially conferred by calreticulin, a protein with both low-affinity/high-capacity and high-affinity/low-capacity Ca2+ binding domains. The effects of over-expression of maize calreticulin on Ca2+ homeostasis are studied in transgenic tobacco cells. |