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| Molecular
and biochemical characterization of Arabidopsis thaliana PI
4-kinase isoforms Jill Stevenson-Paulik PI 4-kinases catalyze the stereospecific phosphorylation of phosphatidylinositol (PtdIns) in the D-4 position of the inositol moiety. The product, PtdIns4P, is the precursor of PtdIns(4,5)P2, a central regulatory compound. PtdIns4P is important for cellular function in its own right, and PI 4-kinase activity has been found in various compartments of the plant cell, such as the cytoskeleton, the plasma membrane, or the nucleus. Compartmentalized pools of PtdIns4P may be synthesized by PI 4-kinase isoforms in different locations of the cell. It is our goal to understand the metabolic roles of PI 4-kinases by characterizing the PI 4-kinase isoforms biochemically and study the enzymes' cellular location. References: Stevenson et al., 1998 |