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Course information for                                  Department of Microbiology  

Introduction to Applied Bioprocessing

                                                          

Course #

BEC(MB)180

Instructor

Dr. José Manuel Bruno-Bárcena

1552A Gardner Hall Addition

North Carolina State University

Raleigh, NC  27695

Phone: 919-513-1495
Fax: 919-513-8235
email: jbbarcen@ncsu.edu
web site: http://www4.ncsu.edu/~jbbarcen

 

Dr.    Driss Elhanafi

Phone: 919-513-8236 email: delhana@gw.fis.ncsu.edu

 

M.S. Matthew Evans

Phone: 919-513-8094 email: mrevans@ncsu.edu

 

M.S. Haiwei Zhang

Phone: 919-513-1699 email: hzhang6@gw.fis.ncsu.edu

 

850 Oval Drive, Suite 195

Raleigh, NC  27695-7928

 

Prerequisites

High school Biology and Chemistry

Class Hours

Office Hours

Lecture - Laboratory– Friday, July 09 – August 3, from 9 am to 3:30 pm Tuesday, 1500-1530 Monday

Credit Hours

2

Restrictions

Who should apply?

  • Applicants should have a strong interest in biotechnology and/or science.
  • Applicants should have completed the necessary prerequisite high school courses.

Course Website

http://www.cifr.ncsu.edu/scibls/

Delivery Format

This is a Summer class. Students are required to attend daily lectures and laboratories during the weeks the course is taught.

Course Description

The course introduces students to basic biomanufacturing tools and experiences, coupled with data analysis, interpretation, and presentation.  The course provides a hands-on learning experience that challenges students to enhance existing skills, learn new information, and apply learning to understand biomanufacturing fundamentals.  The educational environment will emphasize team-based decisions, collaborations and consideration of multiple perspectives.  The course culminates with students cloning and growing cells, expressing, clarifying, purifying, formulating and packaging vials of a recombinant protein. Students successfully completing this experience will understand the fundamentals of biomanufacturing, empowering them to intelligently evaluate biotechnology career options. Through this course, students will apply skills and perform operations that are relevant to the biomanufacturing industries.  Transportation will be provided for field trips to local biomanufacturing facilities.

Technology Requirements

In order to complete the course, all students will be required to have access to an active internet connection.  If you do not have Adobe Acrobat Reader installed on your computer, you will need to go to the following web site and follow the instructions to download a free version.

                     http://www.adobe.com/products/acrobat/readstep2.html

Text Requirements

All required reading material is contained within the course or is available through a World Wide Web link provided within the course content.  At present, all laboratory reading materials will be provided. The class links page is also available as a source of the following references for the module:

 

1. Shuler, M.L., Kargi, F., editors 2002. Bioprocess Engineering: Basic Concepts. Prentice-Hall of India (ISBN0130819085) $127.00

2. Harley-Prescott 2002. Laboratory Exercises in Microbiology, Fifth Edition. The McGraw-Hill Companies. (ISBN0072333456) $70.63

 

These references will also be available in the Reserve Room of the D. H. Hill Library.

Learning Outcomes

At the end of this course, you should be able to:

  • Start, streak, bank, sample, analyze and store cultures
  • Quantify cell populations via microscopy, optical density and plating
  • Safely perform the basic biomanufacturing steps of transformation, cellular growth, induction, cell harvest, homogenization, clarification, column chromatography, buffer exchange, formulation and vialing.
  • Determine and analyze results of laboratory experiments.

Lecture & Laboratory Outlines by Topical Areas

Week 1

Read Chapter 2, An Overview of Biological Basics, Section 2.1 (pp. 11-25), and Sections 2.3-2.4 (pp. 46-54)

Prior to the Unit 1 Lab, review the section of your lab manual entitled ORIENTATION TO THE LABORATORY: RULES OF CONDUCT AND GENERAL SAFETY.

Monday

  • [Orientation]
  • [Campus Tour]
  • Safety
  • Partner Selection
  • Waste Disposal
  • Review & Preview

Tuesday

  • Quiz
  • Measures & Exercises
  • Special Project
  • Aseptic Techniques

Wednesday

Read Exercise 13 Lab Manual

  • Quiz
  • Luria plating
  • Contamination Plates
  • Media Prep and background
  • Review & Preview
  • Review & Preview

Thursday

Read Exercise 19 Lab Manual

  • Quiz
  • Plate inspection
  • Serial dilutions
  • Viability Plating
  • Dry Weight vs Wet Weight
  • Review & Preview

Friday

  • Quiz
  • Dry Weight vs Wet Weight
  • Plate Counting
  • Weekend Homework
  • Review & Preview
  • SCIBLS Poster Training

Week 2

Monday

  • Quiz
  • Contamination plate inspection
  • Notebook Review
  • Spectroscopy
    • Measurement
    • Concentration
    • Linearity
  • Review & Preview

Tuesday

Read Exercise 1 Lab Manual

  • Quiz /Dry weight Analysis
  • Microscopy
    • Pre-made
    • Hemocytometer
    • Sizing
    • Wet Mount
  • Review and Preview

Wednesday

  • Quiz/ Homework Presentation
  • Special Project
  • How to give a presentation
  • Review & Preview
  • SCIBLS – CALS Career Services

Thursday

  • Quiz
  • Biomanufacturing Overview
  • Two Weeks in Review – Student Presentation
  • Review & Preview
  • Company Tour

Friday

Read the following:

Chapter 4, How Cells Work, Sections 4.1 – 4.5 (pp. 105-119)

  • No Quiz
  • Cell banking
  • Dairy Processing Overview
  • Genetics Background
  • Weekend Homework
  • Review & Preview

Week 3

Chapter 6, How Cells Grow, read the following:

Sections 6.1 - 6.2.2  (pp. 155-168)

Monday

Read Exercise 45 Lab Manual

  • Quiz
  • Shake Flask OD Growth
  • Cell bank tests
  • Dairy Pilot Plant Tour
  • GFP Plasmid Purification
  • Review & Preview

Tuesday

Read Biotechnology Explorer™ pGLO™ Bacterial Transformation Kit

  • Quiz
  • Cell Bank tests
  • GFP Transformation
  • Reactor Design
  • Special Project & Presentation
  • Review & Preview

Wednesday

Read Chapter 8, How Cellular Information Is Altered, Sections 8.1 – 8.6 (pp. 219-241)

  • Quiz
  • GFP Flask Growth
  • GFP % Transformation
  • Cell Bank -Student Presentation
  • GFP Expression
  • Notebook Review
  • Review & Preview

Thursday

  • Quiz
  • Protein Quantification
  • GFP % Transformation
  • Cell Recovery
  • Centrifugation
  • Review & Preview

Friday

Read Biotechnology Explorer™ pGLO™ Bacterial Transformation Kit

  • Quiz
  • Poster Project
  • GFP cell disruption & viability
  • Homogenization
  • GFP quantification
  • Review & Preview

Week 4

Monday

  • Quiz
  • Lysis viability reduction
  • Poster work
  • GFP chromatography
  • Chromatography
  • Review & Preview

Tuesday

  • Quiz
  • GFP Filtration
  • Tangential Flow Filtration
  • Formulation and Vialing
  • GFP Vialing
  • Review & Preview

Wednesday

  • Quiz
  • Analytical results
  • Poster Work
  • Course Review
  • BTEC Programs
  • Course Evaluation
  • Review & Preview

Thursday

  • Test
  • Notebook Review
  • [Poster – Deadline]
  • [Poster Presentation Practice]
  • [Final Thoughts]

Friday